Handbook of fluorescent dyes and probes pdf
Bright Ideas for Chemical BiologySmall-molecule fluorescent probes embody an essential facet of chemical biology. The elaboration of these dyes with diverse chemical moieties is enabling the precise interrogation of biochemical and biological systems. The importance of fluorescence-based technologies in chemical biology elicits a necessity to understand the major classes of small-molecule fluorophores. Here, we examine the chemical and photophysical properties of oft-used fluorophores, and highlight classic and contemporary examples in which utility has been built upon these scaffolds. Small fluorescent molecules are indispensable tools for chemical biology, being ubiquitous as biomolecular labels, enzyme substrates, environmental indicators, and cellular stains 1 — Choosing a suitable fluorophore to visualize a biochemical or biological process can be daunting, given the countless molecules available either commercially 13 or through de novo design and synthesis.
SERVA Webinar: Protein Labelling and Staining using Fluorescent Dyes (Deutsch)
10 Tips for Selecting and Using Fluorophores in IF Experiments
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Familarize yourself with your microscope set up
Water-soluble organic fluorophores are widely used as labels in biological systems. Here, we quantify the interaction of 32 common water-soluble dyes with model lipid bilayers to serve as an additional criterion when selecting a dye label. This is an open-access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: L. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Organic fluorophores are ubiquitous tags for a host of biological targets, including cytosolic proteins, membrane proteins, and antibodies.